Resources with keywords: BSL-2
Aloosh M, Saeed HM, Mataseje LF
KPC-producing Klebsiella pneumoniae transmission documented in a long-term care facility, underscoring HCW role in CRE transmission. In the U.S., CRE infections surged 69% in 2025; NDM-producing strains increased 461%. Healthcare workers in ICUs, wound care, and catheter management are at highest occupational exposure risk. Contact precautions, dedicated equipment, and environmental decontamination are the primary control measures. Clinical laboratory staff should handle CRE cultures under BSL-2 containment.
Autochthonous clade Ib transmission confirmed in EU/EEA. Outside Africa, 97% of cases are male, 89% MSM — pattern relevant to clinical triage and ED screening protocols. Laboratory personnel should note that standard dermatopathology laboratory procedures (formalin fixation) adequately inactivate orthopoxviruses; however, unfixed specimens require BSL-2 handling. ECDC recommends JYNNEOS post-exposure prophylaxis for HCWs with unprotected exposure within 4 days.
HCWs caring for mpox patients without adequate PPE (gown, gloves, eye protection, N95 for aerosol-generating procedures) face direct contact transmission risk. All clades (Ia, Ib, IIb) circulating globally. Clade Ib recombinant with Ia/IIb genomic elements detected in February 2026; biological behavior not yet fully characterized. Diagnostic laboratory personnel handling skin lesion specimens must use BSL-2 enhanced precautions. JYNNEOS vaccine recommended for HCWs at occupational risk.
As of 18 May: 10 cases (8 lab-confirmed), 3 deaths. No confirmed U.S. Andes virus cases. Clinical laboratories advised to handle specimens from suspected HPS patients under BSL-2 enhanced precautions (Class II BSC, respirator). Andes virus is NOT a U.S. Select Agent, but laboratory directors should review biosafety risk assessments for specimen processing. Serology (IgM/IgG ELISA) and RT-PCR are diagnostic methods of choice; CDC DHCPP available for testing support.
GVN assessment (3 Apr 2026) concludes BA.3.2 shows antibody escape but no evidence of increased severity or aerosol-generating transmissibility beyond known Omicron characteristics. Standard clinical laboratory biosafety (BSL-2) remains appropriate for routine SARS-CoV-2 specimen processing. Facilities should continue wastewater surveillance as an early-warning tool for variant emergence that could affect HCW exposure levels.
The Training Analysis for a BSL-2 Biomedical Laboratory was created to define all tasks and subtasks in biosafety for all persons working in an international infectious disease detection laboratory. The analysis was done using the Instructional Systems Design (ISD) methodology defined in the publication, ISD From the Ground Up1. The product is a template that can be used as a reference for developing a laboratory-based biosafety training program for BSL-2 biomedical laboratories or as a base for the development of training for microbiological and containment laboratories. This template was originally used in the design of a competency training program. Application of the training analysis in a competency training program is more fully described in “Quantifying Competency in Biosafety: Application of the Instructional Systems Design (ISD) to Biosafety and Laboratory Biosecurity”, Delarosa, P., et al., Applied Biosafety 16:3, 2011. However, this type of analysis can be applied to any training design and can be readily applied to the development of both didactic and practical training materials.
Target Audience: All persons designing biosafety training. Persons interested in competency training in Biosafety
This is a “do it yourself” methodology for the development of biosafety training that incorporates biosafety tasks into working procedures and practices in the laboratory. The goal of the training design is to accurately define all biosafety training that an individual needs to safely complete their work and establish a method for practical integration of biosafety procedures on the job.
BSL2 case studies for use in training to reemphasize key lessons taught in course lecture. Also provides an interactive exercises for students to practice and used what was learned in the biosafety training class.
Target Audience: Laboratory Researchers
At the end of training students will be able to…
demonstrate their awareness with key biosafety risk management practices.
You must have access to the my.ABSA.org website.
